6HJL
Affimer:BclxL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9174 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.270, 87.290, 112.180 |
| Unit cell angles | 90.00, 96.18, 90.00 |
Refinement procedure
| Resolution | 55.760 - 2.200 |
| R-factor | 0.21753 |
| Rwork | 0.215 |
| R-free | 0.26664 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.548 |
| Data reduction software | xia2 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.760 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.240 |
| Number of reflections | 62008 | |
| <I/σ(I)> | 4.1 | |
| Completeness [%] | 98.1 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | MPD |
