6HJC

Crystal structure of Loei River virus GP1 glycoprotein at pH 8.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	P 41 21 2
Unit cell lengths	60.540, 60.540, 96.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.125 - 2.510
R-factor	0.2089
Rwork	0.207
R-free	0.23820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zjf
RMSD bond length	0.002
RMSD bond angle	0.420
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.125	2.550
High resolution limit [Å]	2.510	2.510
Rmerge	0.112
Number of reflections	6515
<I/σ(I)>	15.4
Completeness [%]	99.2
Redundancy	11.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	5.3 mg/ml protein, 10% w/v ethanol, and 1.5 M NaCl