6HJ4

Crystal structure of Whitewater Arroyo virus GP1 glycoprotein at pH 7.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97950
Spacegroup name	P 63 2 2
Unit cell lengths	106.660, 106.660, 74.810
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.473 - 2.430
R-factor	0.2217
Rwork	0.220
R-free	0.25220
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.549
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.000	2.490
High resolution limit [Å]	2.430	2.430
Rmerge	0.068	3.163
Number of reflections	15417
<I/σ(I)>	22.8
Completeness [%]	99.9
Redundancy	18.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	1.0 mg/ml protein, 0.1 M HEPES-Na pH 7.5, and 0.05 M cadmium sulphate
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	1.0 mg/ml protein, 0.1 M HEPES-Na pH 7.5, and 0.05 M cadmium sulphate