6HIF

Kuenenia stuttgartiensis hydrazine dehydrogenase complex

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.95376
Spacegroup name	P 31
Unit cell lengths	211.860, 211.860, 398.570
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	93.710 - 2.800
R-factor	0.222
Rwork	0.221
R-free	0.23838
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4n4j
RMSD bond length	0.003
RMSD bond angle	0.947
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	185.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.111	0.745
Rmeas	0.147	0.994
Number of reflections	452098	49339
<I/σ(I)>	6.67	1.2
Completeness [%]	96.6	98.8
Redundancy	2
CC(1/2)	0.989	0.457

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1+1 ul hanging drops against a reservoir solution containing 8-9% (w/v) polyethylene glycol 4000, 0.25 M (NH4)2SO4.