6HHS
MamM CTD E289D - Cadmium form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 83.599, 94.545, 107.490 |
| Unit cell angles | 90.00, 91.76, 90.00 |
Refinement procedure
| Resolution | 47.270 - 2.700 |
| R-factor | 0.25167 |
| Rwork | 0.250 |
| R-free | 0.29043 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w5x |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.346 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.270 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.273 | |
| Number of reflections | 22985 | 2990 |
| <I/σ(I)> | 5.7 | 1.7 |
| Completeness [%] | 99.3 | 97.5 |
| Redundancy | 6.8 | 6.9 |
| CC(1/2) | 0.984 | 0.639 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M (NH4)2SO4, 0.1M BIS-TRIS pH=6.5, 0.1M NaCl, 1.7mM CdCl2 |
