6HHE
Crystal structure of the medfly Odorant Binding Protein CcapOBP22/CcapOBP69a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-11 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 43.030, 43.030, 136.372 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.036 - 1.516 |
| R-factor | 0.1944 |
| Rwork | 0.193 |
| R-free | 0.22060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ogn |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.327 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | BALBES |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.030 | 1.540 |
| High resolution limit [Å] | 1.516 | 1.516 |
| Rmerge | 0.060 | 0.450 |
| Rpim | 0.031 | 0.241 |
| Number of reflections | 20882 | 1017 |
| <I/σ(I)> | 16.7 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.8 | 8.1 |
| CC(1/2) | 0.996 | 0.924 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 297 | 100 mM MES pH 6.5, 1.6 M Ammonium Sulfate, 10% v/v 1,4-Dioxane |
