6HH2

Rab29 small GTPase bound to GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.98
Spacegroup name	P 31 2 1
Unit cell lengths	71.960, 71.960, 86.348
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	62.319 - 1.449
R-factor	0.1661
Rwork	0.165
R-free	0.18430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6hdu
RMSD bond length	0.017
RMSD bond angle	1.629
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3211: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.350	1.470
High resolution limit [Å]	1.449	1.449
Rmerge	0.052	1.631
Rmeas	0.055
Rpim	0.017	0.585
Number of reflections	46405
<I/σ(I)>	27.4	1.4
Completeness [%]	99.8
Redundancy	9.7
CC(1/2)		0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288	2M NaCl 0.1M MES pH 6.5