6HGR
Crystal Structure of Human APRT wild type in complex with IMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.585, 47.625, 47.846 |
| Unit cell angles | 76.43, 69.24, 61.29 |
Refinement procedure
| Resolution | 44.600 - 1.520 |
| R-factor | 0.1709 |
| Rwork | 0.170 |
| R-free | 0.19310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5DE6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.301 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.600 | 1.570 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.051 | 0.670 |
| Number of reflections | 46425 | |
| <I/σ(I)> | 11.9 | 1.7 |
| Completeness [%] | 92.5 | 89.9 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | NaOAc, PEG4000, Glycerol, Tris |
