6HFO
Human Hsp90 co-chaperone TTC4 C-domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97856 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.680, 42.800, 43.090 |
| Unit cell angles | 90.00, 93.63, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.650 |
| R-factor | 0.16031 |
| Rwork | 0.159 |
| R-free | 0.18947 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6hfm |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.163 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 1.750 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.044 | 0.577 |
| Number of reflections | 20421 | |
| <I/σ(I)> | 12.8 | 1.9 |
| Completeness [%] | 97.7 | 97.9 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.4 M Na/K phosphate pH 8.2 |
