6HF4

The structure of BoMan26B, a GH26 beta-mannanase from Bacteroides ovatus, complexed with G1M4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.96862
Spacegroup name	P 21 21 21
Unit cell lengths	50.122, 68.363, 94.993
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.496 - 1.781
R-factor	0.1794
Rwork	0.178
R-free	0.21040
RMSD bond length	0.007
RMSD bond angle	0.869
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.500	1.850
High resolution limit [Å]	1.780	1.780
Number of reflections	57371
<I/σ(I)>	6.3
Completeness [%]	95.1
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	0.1 M bis-tris, 20% PEG6000, 0.2 M NaCl, 50 mM CaCl2, 10 mM di-galactosyl-mannopentaose