6HEM

Structure of the C-terminal domain of USP25 (748-1048)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-04
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	55.957, 78.288, 84.909
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.557 - 1.720
R-factor	0.1824
Rwork	0.181
R-free	0.20870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.845
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AMPLE
Refinement software	PHENIX ((1.13_2998))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.560	1.780
High resolution limit [Å]	1.720	1.720
Rmerge	0.071	0.751
Rmeas	0.080	0.852
Number of reflections	40292	3983
<I/σ(I)>	13.5	2.5
Completeness [%]	99.9	99.9
Redundancy	4.6	4.4
CC(1/2)	0.996	0.755

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	20% (w/v) PEG 4000, 600 mM sodium chloride, 100 mM MES pH 6.5