6HEG

Crystal structure of Escherichia coli DEAH/RHA helicase HrpB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	48.550, 106.372, 180.005
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.186 - 3.019
R-factor	0.2106
Rwork	0.208
R-free	0.26290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xau
RMSD bond length	0.006
RMSD bond angle	1.031
Data reduction software	XDS (BUILT 20180409)
Data scaling software	Aimless (0.7.1)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX ((1.14rc1_3177: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.194	3.127
High resolution limit [Å]	3.019	3.019
Rmerge	0.184	0.789
Rmeas	0.201	0.860
Rpim	0.080	0.340
Number of reflections	19075	1863
<I/σ(I)>	8.16	2.16
Completeness [%]	99.9	100
Redundancy	6.4	6.3
CC(1/2)	0.990	0.777

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	Na Citrate 0.12M PEG 2000 MME 20% (w/v)