6HDV
The crystal structure of intact afifavidin apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 161.986, 161.986, 46.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.930 - 2.160 |
| R-factor | 0.19752 |
| Rwork | 0.195 |
| R-free | 0.25308 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ew1 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.709 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.930 | 2.230 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Number of reflections | 32236 | |
| <I/σ(I)> | 9.2 | |
| Completeness [%] | 94.5 | |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1.4M Ammonium Sulfate, 0.1M Bis-Tris pH 7.0, 1% PEG 2000 |
