6HD3
Common mode of remodeling AAA ATPases p97/CDC48 by their disassembly cofactors ASPL/PUX1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 146.266, 146.266, 173.133 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.880 - 2.800 |
| R-factor | 0.221 |
| Rwork | 0.219 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s3s |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.790 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.900 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmeas | 0.024 | 0.258 |
| Number of reflections | 27532 | 2673 |
| <I/σ(I)> | 11.44 | 0.98 |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 12.84 | |
| CC(1/2) | 0.998 | 0.628 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 293 | 1.8M Na/K phosphate pH 6.9; 50mM KCL |
