6HCT
Crystal structure of Archeoglobus fulgidus L7Ae bound to its cognate UTR k-turn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.91962 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 142.140, 142.140, 166.798 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.070 - 3.091 |
| R-factor | 0.1891 |
| Rwork | 0.187 |
| R-free | 0.23660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bw0 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.621 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.400 | 3.140 |
| High resolution limit [Å] | 3.090 | 3.090 |
| Rmerge | 0.086 | 0.914 |
| Rpim | 0.028 | 0.291 |
| Number of reflections | 16002 | 785 |
| <I/σ(I)> | 13.9 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.6 | 10.5 |
| CC(1/2) | 0.998 | 0.382 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 280 | 0.01 M Magnesium Acetate, 0.05 M MES pH 5.6, 2.5 M Ammonium Sulfate |
