6HCT

Crystal structure of Archeoglobus fulgidus L7Ae bound to its cognate UTR k-turn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.91962
Spacegroup name	I 4 2 2
Unit cell lengths	142.140, 142.140, 166.798
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.070 - 3.091
R-factor	0.1891
Rwork	0.187
R-free	0.23660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bw0
RMSD bond length	0.011
RMSD bond angle	1.621
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.400	3.140
High resolution limit [Å]	3.090	3.090
Rmerge	0.086	0.914
Rpim	0.028	0.291
Number of reflections	16002	785
<I/σ(I)>	13.9
Completeness [%]	100.0	100
Redundancy	10.6	10.5
CC(1/2)	0.998	0.382

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	280	0.01 M Magnesium Acetate, 0.05 M MES pH 5.6, 2.5 M Ammonium Sulfate