6HCN

Adenovirus Type 5 Fiber Knob protein at 1.49A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.96859
Spacegroup name	P 21 21 21
Unit cell lengths	102.160, 102.440, 77.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.560 - 1.490
R-factor	0.21201
Rwork	0.211
R-free	0.23305
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1knb
RMSD bond length	0.011
RMSD bond angle	1.534
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.560	1.530
High resolution limit [Å]	1.490	1.490
Rmerge	0.134	1.838
Rmeas	0.159	2.183
Number of reflections	131951	9638
<I/σ(I)>	7.1
Completeness [%]	99.8	99.9
Redundancy	6.6	6.3
CC(1/2)	0.983	0.565

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	DL-Malic acid, MES monohydrate, Tris, pH 7.0 (condition D4 of PACT premier suite from Molecular Dimensions)