Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-22 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.174, 99.158, 57.387 |
| Unit cell angles | 90.00, 92.38, 90.00 |
Refinement procedure
| Resolution | 32.010 - 1.900 |
| R-factor | 0.16936 |
| Rwork | 0.167 |
| R-free | 0.20736 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3DLO |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.114 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 5.160 | 1.900 |
| Rmerge | 0.066 | 0.027 | 0.762 |
| Rmeas | 0.074 | 0.031 | 0.867 |
| Rpim | 0.034 | 0.014 | 0.408 |
| Number of reflections | 38249 | 1957 | 1886 |
| <I/σ(I)> | 8.4 | ||
| Completeness [%] | 99.9 | 99.3 | 100 |
| Redundancy | 4.5 | 4.7 | 4.3 |
| CC(1/2) | 0.998 | 0.746 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1M BIS-TRIS PH5.5, 0.2M AMMONIUM ACETATE, 25%PEG3350. |
