6HBT

The structure of the G. violaceus guanidine II riboswitch P2 stem-loop with arcaine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9197
Spacegroup name	H 3 2
Unit cell lengths	55.460, 55.460, 135.040
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.730 - 1.660
R-factor	0.2066
Rwork	0.206
R-free	0.21380
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.195
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3228: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.730	1.690
High resolution limit [Å]	1.660	1.660
Rmerge	0.056	1.316
Rpim	0.028	0.647
Number of reflections	17902	463
<I/σ(I)>	19.6	1.3
Completeness [%]	98.9	97.06
Redundancy	4.79	5
CC(1/2)	0.999	0.571

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	0.01 M Magnesium Acetate, 0.05 M MES pH 5.6, 2.5 M Ammonium Sulfate