6HBT
The structure of the G. violaceus guanidine II riboswitch P2 stem-loop with arcaine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-05 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9197 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 55.460, 55.460, 135.040 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.730 - 1.660 |
| R-factor | 0.2066 |
| Rwork | 0.206 |
| R-free | 0.21380 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.195 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3228: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.730 | 1.690 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.056 | 1.316 |
| Rpim | 0.028 | 0.647 |
| Number of reflections | 17902 | 463 |
| <I/σ(I)> | 19.6 | 1.3 |
| Completeness [%] | 98.9 | 97.06 |
| Redundancy | 4.79 | 5 |
| CC(1/2) | 0.999 | 0.571 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.01 M Magnesium Acetate, 0.05 M MES pH 5.6, 2.5 M Ammonium Sulfate |
