6HBI

SCAPHARCA DIMERIC HEMOGLOBIN, MUTANT T72V, DEOXY FORM

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1996-03
Detector	RIGAKU
Spacegroup name	C 2 2 21
Unit cell lengths	91.900, 44.410, 144.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.188
Rwork	0.188
R-free	0.23800
Structure solution method	ISOMORPHOUS MOLECULAR REPLACEMENT
Starting model (for MR)	4sdh
RMSD bond length	0.010
RMSD bond angle	17.900 *
Data reduction software	RIGAKU
Data scaling software	RIGAKU
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.800
Rmerge	0.089
Total number of observations	140113 *
Number of reflections	24855
Completeness [%]	90.2
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7.5 *		Royer Junior, W.E., (1994) J.Mol.Biol., 235, 657. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	hemoglobin	40-50 (mg/ml)
2	1	1	phosphate	2.1-2.3 (M)