6HAS

Crystal Structure of the small subunit-like domain of Rubisco activase from Nostoc sp. (strain PCC 7120)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-03
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.96500
Spacegroup name	P 61
Unit cell lengths	59.732, 59.732, 83.305
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.380
R-factor	0.155
Rwork	0.153
R-free	0.18940
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.549
Data reduction software	XDS (VERSION January 10, 2014)
Data scaling software	Aimless (0.1.27)
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.950	43.950	1.400
High resolution limit [Å]	1.380	7.560	1.380
Rmerge	0.065	0.042	0.922
Rmeas	0.072	0.046	1.037
Rpim	0.030	0.018	0.459
Total number of observations	174770
Number of reflections	34504	226	1679
<I/σ(I)>	12.5
Completeness [%]	99.6	98.5	97.5
Redundancy	5.1	5.2	4.6
CC(1/2)	0.998	0.998	0.538

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	277	22% PEG-3350, 50 mM MES-NaOH pH 6.0