6HAS
Crystal Structure of the small subunit-like domain of Rubisco activase from Nostoc sp. (strain PCC 7120)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-03 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.96500 |
| Spacegroup name | P 61 |
| Unit cell lengths | 59.732, 59.732, 83.305 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.380 |
| R-factor | 0.155 |
| Rwork | 0.153 |
| R-free | 0.18940 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.549 |
| Data reduction software | XDS (VERSION January 10, 2014) |
| Data scaling software | Aimless (0.1.27) |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.950 | 43.950 | 1.400 |
| High resolution limit [Å] | 1.380 | 7.560 | 1.380 |
| Rmerge | 0.065 | 0.042 | 0.922 |
| Rmeas | 0.072 | 0.046 | 1.037 |
| Rpim | 0.030 | 0.018 | 0.459 |
| Total number of observations | 174770 | ||
| Number of reflections | 34504 | 226 | 1679 |
| <I/σ(I)> | 12.5 | ||
| Completeness [%] | 99.6 | 98.5 | 97.5 |
| Redundancy | 5.1 | 5.2 | 4.6 |
| CC(1/2) | 0.998 | 0.998 | 0.538 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 277 | 22% PEG-3350, 50 mM MES-NaOH pH 6.0 |
