6HAP
Adenylate kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-09-25 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5417 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 73.560, 76.840, 86.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.490 - 2.700 |
| R-factor | 0.2263 |
| Rwork | 0.224 |
| R-free | 0.27760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jzk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 2.291 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1-2575_1692: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.490 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 7058 | 696 |
| <I/σ(I)> | 16.48 | |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 5.8 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 10% PEG 3350 0.05M Tris pH=5.5 |
