6HAN
MamM CTD H264E-E289H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.589, 66.643, 113.294 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.750 - 2.600 |
| R-factor | 0.21273 |
| Rwork | 0.210 |
| R-free | 0.25902 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w5x |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.116 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.750 | 2.710 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.150 | |
| Number of reflections | 15869 | 1849 |
| <I/σ(I)> | 10.5 | 1.4 |
| Completeness [%] | 99.6 | 97.3 |
| Redundancy | 8.4 | 6.5 |
| CC(1/2) | 0.997 | 0.595 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M AmSu, 0.1M HEPES pH=7.5, 25% PEG 3350, 1.7mM MnCl2 |
