6HA6

Factor Inhibiting HIF (FIH) in complex with zinc, NOG and TRPV3 (220-246)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-02
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 41 21 2
Unit cell lengths	87.080, 87.080, 149.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.575 - 1.980
R-factor	0.1818
Rwork	0.181
R-free	0.20280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h2k
RMSD bond length	0.003
RMSD bond angle	0.591
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.680	2.010
High resolution limit [Å]	1.980	1.980
Rmeas	0.085	2.821
Number of reflections	40834	2001
<I/σ(I)>	18	1.4
Completeness [%]	100.0	100
Redundancy	19	20.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M Hepes pH = 7.5, 1.7 M ammonium sulfate, 5.5% PEG400