6H9N

Complex of the periplasmic domains of bacterial cell division proteins FtsQ and FtsB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.92819
Spacegroup name	P 41 21 2
Unit cell lengths	93.785, 93.785, 106.392
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.994 - 2.600
R-factor	0.2243
Rwork	0.223
R-free	0.25190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vh1
RMSD bond length	0.009
RMSD bond angle	1.162
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.082	1.702
Rpim	0.024	0.477
Number of reflections	15037	2155
<I/σ(I)>	22.6	2.1
Completeness [%]	99.5	99.5
Redundancy	12.8	13.4
CC(1/2)	0.999	0.821

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.15 M potassium thiocyanate, 20 % PEG 550 MME, 0.1 M sodium cacodylate pH 6.5