6H9M
Coiled-coil domain-containing protein 90B residues 43-125 from Homo sapiens fused to a GCN4 adaptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-30 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.07030 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 36.009, 298.178, 29.297 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.270 - 2.100 |
| R-factor | 0.23239 |
| Rwork | 0.231 |
| R-free | 0.25728 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5apq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.338 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.300 | 2.230 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.089 | 0.867 |
| Number of reflections | 19522 | |
| <I/σ(I)> | 13.4 | 1.89 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 6.27 | 6.46 |
| CC(1/2) | 0.999 | 0.835 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | Molecular Dimensions Morpheus Screen, Well F2 |
