6H9H

Csf5, CRISPR-Cas type IV Cas6 crRNA endonuclease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.972
Spacegroup name	P 21 21 21
Unit cell lengths	85.563, 100.989, 101.933
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.250 - 1.750
R-factor	0.2135
Rwork	0.212
R-free	0.23710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.125
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.250	1.810
High resolution limit [Å]	1.750	1.750
Rmerge	0.074
Rpim	0.090
Number of reflections	89451
<I/σ(I)>	20.32
Completeness [%]	99.9
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.18 M di-ammonium tartrate, 18% w/v PEG3350, 3% glycerol