6H96
AlbA-albicidin complex, albicidin resistance protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-16 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.07227 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 54.142, 123.181, 161.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.370 - 1.550 |
| R-factor | 0.1895 |
| Rwork | 0.189 |
| R-free | 0.20730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Alba SeMet |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.692 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.370 | 1.605 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.019 | 0.305 |
| Number of reflections | 77989 | |
| <I/σ(I)> | 16.72 | |
| Completeness [%] | 99.5 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.2-0.4 M Ammonium Sulfate, 0.8-1.2 M Lithium Sulfate, 0.1 M sodium citrate tribasic |
