6H94
T16A variant of beta-phosphoglucomutase from Lactococcus lactis with phosphate and TRIS bound in an open conformer to 1.5 A.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-25 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.010, 53.190, 89.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.770 - 1.490 |
| R-factor | 0.19302 |
| Rwork | 0.192 |
| R-free | 0.21031 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wfa |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.490 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.770 | 1.520 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.040 | 2.232 |
| Rmeas | 0.041 | 2.323 |
| Rpim | 0.012 | 0.639 |
| Number of reflections | 39950 | 1963 |
| <I/σ(I)> | 25.4 | |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 12.7 | 12.8 |
| CC(1/2) | 1.000 | 0.596 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 291 | 24-34% PEG 4000, 200 mM sodium acetate, 50 mM TRIS pH 7.2 |
