6H8U
Beta-phosphoglucomutase from Lactococcus lactis in an open conformer to 1.9 A.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-23 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.150, 53.640, 81.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.450 - 1.900 |
| R-factor | 0.21214 |
| Rwork | 0.211 |
| R-free | 0.23929 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2whe |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.483 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.450 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.118 | 0.908 |
| Rmeas | 0.154 | 1.147 |
| Rpim | 0.073 | 0.544 |
| Number of reflections | 18801 | 1358 |
| <I/σ(I)> | 9.6 | 1.6 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 4.3 | 4.2 |
| CC(1/2) | 0.996 | 0.604 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 291 | 24-34% PEG 4000, 200 mM sodium acetate, 50 mM TRIS pH 7.5 |
