6H8Q

Structural basis for Scc3-dependent cohesin recruitment to chromatin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-21
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.966
Spacegroup name	P 21 21 2
Unit cell lengths	296.240, 110.020, 115.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.631
R-factor	0.2854
Rwork	0.283
R-free	0.31850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uvk 4uvl
RMSD bond length	0.002
RMSD bond angle	0.451
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.800
High resolution limit [Å]	3.600	3.600
Rmerge	0.058
Number of reflections	20963
<I/σ(I)>	11.9
Completeness [%]	91.4
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	10% PEG 8000, 0.1M Bis-TRIS, pH 6.5