6H8M
Crystal structure of the third SRCR domain of Murine Neurotrypsin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-09 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.983 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.752, 62.902, 84.526 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.580 - 1.700 |
| R-factor | 0.19122 |
| Rwork | 0.190 |
| R-free | 0.21306 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1by2 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.699 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.600 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.042 | 2.840 |
| Number of reflections | 34576 | 1785 |
| <I/σ(I)> | 15.5 | 0.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.9 | 6.9 |
| CC(1/2) | 0.999 | 0.434 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 16% PEG 3350, 0.1 M Tri-soidium citrate |
