6H8E
Truncated derivative of the C-terminal domain of the TssA component of the type VI secretion system from Burkholderia cenocepacia
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-03 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97943 |
| Spacegroup name | P 62 |
| Unit cell lengths | 65.280, 65.280, 66.140 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.970 - 2.350 |
| R-factor | 0.19543 |
| Rwork | 0.193 |
| R-free | 0.23694 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6h8f |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.432 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.970 | 2.410 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.094 | 0.713 |
| Rpim | 0.037 | 0.286 |
| Number of reflections | 6747 | |
| <I/σ(I)> | 17.1 | |
| Completeness [%] | 99.9 | |
| Redundancy | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.18 M tri-ammonium citrate, 20% (w/v) PEG3350 |
