6H87
MamM CTD D249N - Zinc form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.975 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 53.137, 53.137, 64.465 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.020 - 1.500 |
| R-factor | 0.19122 |
| Rwork | 0.189 |
| R-free | 0.22706 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w5x |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.034 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0216) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.020 | 1.520 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.050 | |
| Number of reflections | 17417 | |
| <I/σ(I)> | 31.3 | 2.9 |
| Completeness [%] | 99.8 | 95.3 |
| Redundancy | 12.6 | 10.7 |
| CC(1/2) | 1.000 | 0.933 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Tris pH=8.5, 0.3M MgFormate, 1.7mM ZnCl2 |
