6H86

Rebuilt and re-refined PDB entry 4R3Q: Crystal structure of SYCE3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-30
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9796
Spacegroup name	H 3
Unit cell lengths	75.576, 75.576, 101.511
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.661 - 1.901
R-factor	0.1934
Rwork	0.192
R-free	0.21670
RMSD bond length	0.016
RMSD bond angle	1.232
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((1.13_2997: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.900	1.900
Number of reflections	16498
<I/σ(I)>	22
Completeness [%]	96.9
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1M Citric acid, 7% 2-propanol, 1% PEG 20000, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K