6H7H

Crystal structure of redox-sensitive phosphoribulokinase (PRK) from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9395
Spacegroup name	I 41
Unit cell lengths	116.296, 116.296, 106.812
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.760 - 2.471
R-factor	0.2292
Rwork	0.226
R-free	0.28110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6h7g
RMSD bond length	0.011
RMSD bond angle	1.204
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.230	2.580
High resolution limit [Å]	2.470	2.470
Rmerge	0.091	0.435
Number of reflections	24824	3107
<I/σ(I)>	11.2	2.3
Completeness [%]	97.6	99.6
Redundancy	6.9	6.5
CC(1/2)	0.997	0.947

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	1.5 M sodium malonate, pH 5.0