6H7G

Crystal structure of redox-sensitive phosphoribulokinase (PRK) from the green algae Chlamydomonas reinhardtii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-15
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.240
Spacegroup name	P 21 21 21
Unit cell lengths	77.679, 83.549, 133.146
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.859 - 2.600
R-factor	0.2291
Rwork	0.227
R-free	0.26190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5b3f
RMSD bond length	0.004
RMSD bond angle	0.915
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.380	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.076	0.770
Number of reflections	27230	3280
<I/σ(I)>	13.6	1.7
Completeness [%]	99.6	100
Redundancy	5.1	5.3
CC(1/2)	0.997	0.743

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	22 % w/v PEG MME 5K, 0.1 M MES, pH 6.5, 0.2 M ammonium sulfate