6H7D

Crystal Structure of A. thaliana Sugar Transport Protein 10 in complex with glucose in the outward occluded state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9686
Spacegroup name	P 1 21 1
Unit cell lengths	51.680, 92.460, 66.800
Unit cell angles	90.00, 109.37, 90.00

Refinement procedure

Resolution	63.018 - 2.400
R-factor	0.2057
Rwork	0.203
R-free	0.26820
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS (Jan 26, 2018)
Data scaling software	XSCALE (Jan 26, 2018)
Phasing software	PHASER (2.8.1)
Refinement software	PHENIX (1.13rc1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	63.018	63.018	2.500
High resolution limit [Å]	2.400	20.000	2.400
Rmerge	0.282	0.083	2.155
Rmeas	0.306	0.096	2.343
Total number of observations	153232
Number of reflections	23248	46	2656
<I/σ(I)>	4.9	14.59	0.89
Completeness [%]	99.8	97.9	99.8
Redundancy	6.591	4.826	6.646
CC(1/2)	0.991	0.999	0.348

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	4.5	293	0.1M NaCitrate pH 4.5, 0.01 M Ammonium dihydrogen phosphate, 5% DMSO, 30% PEG400