6H7A

Structure of Leishmania PABP1 (domain J).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9760
Spacegroup name	P 65
Unit cell lengths	82.970, 82.970, 46.080
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.158 - 2.030
R-factor	0.2027
Rwork	0.201
R-free	0.22490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pth
RMSD bond length	0.002
RMSD bond angle	0.423
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.103
High resolution limit [Å]	2.030	2.030
Rmerge	0.128	1.821
Rmeas	0.132	1.868
Rpim	0.030	0.419
Number of reflections	11824	1175
<I/σ(I)>	14.98
Completeness [%]	99.8	99.91
Redundancy	19.9	19.8
CC(1/2)	0.999	0.683

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M ammonium sulphate, 0.1 M Sodium cacodylate at pH 6.5, 30% PEG8000