6H6J
Carbomonoxy murine neuroglobin Gly-loop mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-18 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 74.859, 74.859, 77.229 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.640 - 2.600 |
| R-factor | 0.19663 |
| Rwork | 0.194 |
| R-free | 0.24570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w92 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.873 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.600 | 2.750 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.194 | |
| Number of reflections | 7550 | |
| <I/σ(I)> | 3.1 | |
| Completeness [%] | 90.4 | |
| Redundancy | 6 | |
| CC(1/2) | 0.900 | 0.104 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.6 M ammonium sulfate, 0.1 M tris pH 7.5, 4% PEG 1000 |
