6H6G
Crystal Structure of TcdB2-TccC3 without hypervariable C-terminal region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-06 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97794 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 231.330, 231.330, 141.970 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.639 - 3.004 |
| R-factor | 0.2175 |
| Rwork | 0.216 |
| R-free | 0.25310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o9x |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.568 |
| Data reduction software | XDS (BUILT=20160617) |
| Data scaling software | XSCALE (BUILT=20160617) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.575 | |
| Rmeas | 0.590 | |
| Rpim | 0.129 | |
| Number of reflections | 87208 | |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 99.9 | |
| Redundancy | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M tri-sodium citrate pH 5.5, 10 % PEG 8000, 10 % ethylenglycol |
