6H5U
MamM CTD H264E - Zinc form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97624 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.049, 55.648, 63.767 |
| Unit cell angles | 90.00, 92.14, 90.00 |
Refinement procedure
| Resolution | 47.030 - 2.000 |
| R-factor | 0.17238 |
| Rwork | 0.170 |
| R-free | 0.22053 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w5x |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.467 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.030 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.083 | |
| Rmeas | 0.087 | |
| Rpim | 0.025 | |
| Number of reflections | 20715 | |
| <I/σ(I)> | 23.2 | 13.9 |
| Completeness [%] | 99.8 | 96.3 |
| Redundancy | 11.8 | |
| CC(1/2) | 0.997 | 0.993 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Ammonium sulfate, 0.1M BIS-TRIS pH=6.5, 25% PEG 3350, 1.7 mM ZnCl2 |
