6H5M
MamM CTD H264E - Zinc form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97624 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 53.273, 53.273, 61.418 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.140 - 1.600 |
| R-factor | 0.21468 |
| Rwork | 0.212 |
| R-free | 0.25520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w5x |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.617 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0171) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.140 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.061 | |
| Rmeas | 0.063 | |
| Rpim | 0.017 | |
| Number of reflections | 13738 | |
| <I/σ(I)> | 17.5 | 1.3 |
| Completeness [%] | 99.7 | 92.3 |
| Redundancy | 12.7 | 11.5 |
| CC(1/2) | 0.999 | 0.640 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.5M MgFormate, 0.1M HEPES pH=7.5, 1.7 mM ZnCl2 |
