Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.822, 72.256, 122.313 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.060 - 2.000 |
| R-factor | 0.196 |
| Rwork | 0.193 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4YIB |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.963 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.060 | 2.070 |
| High resolution limit [Å] | 1.955 | 2.000 |
| Rmerge | 0.063 | |
| Number of reflections | 40499 | |
| <I/σ(I)> | 16.4 | 1.47 |
| Completeness [%] | 98.6 | 94.2 |
| Redundancy | 7.74 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 33% (W/V) PEG 6000, 0.01 M TRI SODIUM CITRATE., PH 7.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291K |
