6H4K

Structure of the Usp25 C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-20
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9790
Spacegroup name	P 43 21 2
Unit cell lengths	87.008, 87.008, 86.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.504 - 2.050
R-factor	0.1931
Rwork	0.192
R-free	0.22240
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	0.855
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.500	2.110
High resolution limit [Å]	2.050	2.050
Rpim	0.024	0.759
Number of reflections	21553	1608
<I/σ(I)>	19.4	1.4
Completeness [%]	100.0	99.7
Redundancy	49.4	41
CC(1/2)	0.999	0.512

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Tris pH 8.5 22% w/v PEG4000 0.2M SrCl2