6H4D
Crystal structure of RsgA from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-11 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 146.405, 146.405, 146.405 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.297 - 2.900 |
| R-factor | 0.25 |
| Rwork | 0.248 |
| R-free | 0.28650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rcn |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.974 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.800 | 48.800 | 3.080 |
| High resolution limit [Å] | 2.900 | 8.700 | 2.900 |
| Rmerge | 0.110 | 0.045 | 1.395 |
| Rmeas | 0.114 | 0.047 | 1.435 |
| Rpim | 0.027 | 0.012 | 0.334 |
| Total number of observations | 214545 | 8595 | 35649 |
| Number of reflections | 12464 | 553 | 1955 |
| <I/σ(I)> | 20.4 | 60 | 2.5 |
| Completeness [%] | 100.0 | 99.6 | 100 |
| Redundancy | 17.2 | 15.5 | 18.2 |
| CC(1/2) | 0.999 | 0.999 | 0.750 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 30% Polyacrylic acid 5100 sodium salt, 0.1 M Hepes pH 7.5, 5% PEG 200, 0.02 M Magnesium chloride |
