6H49

A polyamorous repressor: deciphering the evolutionary strategy used by the phage-inducible chromosomal islands to spread in nature.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97623
Spacegroup name	C 1 2 1
Unit cell lengths	132.538, 34.553, 36.990
Unit cell angles	90.00, 95.96, 90.00

Refinement procedure

Resolution	33.640 - 1.800
R-factor	0.17363
Rwork	0.172
R-free	0.21582
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.968
Data reduction software	xia2
Data scaling software	Aimless (0.5.27)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	33.690	33.690	1.850
High resolution limit [Å]	1.800	8.050	1.800
Rmerge	0.208	0.160	0.208
Rmeas	0.237	0.177	0.227
Rpim	0.094	0.073	0.901
Number of reflections	15780	200	1169
<I/σ(I)>	12.4
Completeness [%]	100.0	99.1	100
Redundancy	6.3	5.8	6.2
CC(1/2)	0.984	0.952	0.481

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294.15	0.2 M Lithium Sulfate, 0.1M Bis-Tris 5.5 pH, 25% PEG3350