6H3X

Oropouche Virus Glycoprotein Gc Head Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-27
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980000
Spacegroup name	H 3 2
Unit cell lengths	104.638, 104.638, 133.504
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.898 - 2.092
R-factor	0.2058
Rwork	0.203
R-free	0.23330
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.613
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.500	2.150
High resolution limit [Å]	2.090	2.090
Rmerge	0.090	0.710
Rmeas	0.090	0.750
Rpim	0.030	0.230
Number of reflections	16679	1159
<I/σ(I)>	13.4	1.7
Completeness [%]	99.1	89.2
Redundancy	11.1	9.6
CC(1/2)	1.000	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	1.0 uL of 20.0 mg/mL OROV Gc head domain in 20 mM Tris-Cl pH 8.0, 150 mM NaCl was added to 1.0 uL of reservoir solution containing 0.5 M lithium sulfate, 15% w/v PEG 8K