6H3X
Oropouche Virus Glycoprotein Gc Head Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-27 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.980000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 104.638, 104.638, 133.504 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.898 - 2.092 |
| R-factor | 0.2058 |
| Rwork | 0.203 |
| R-free | 0.23330 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.613 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.500 | 2.150 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.090 | 0.710 |
| Rmeas | 0.090 | 0.750 |
| Rpim | 0.030 | 0.230 |
| Number of reflections | 16679 | 1159 |
| <I/σ(I)> | 13.4 | 1.7 |
| Completeness [%] | 99.1 | 89.2 |
| Redundancy | 11.1 | 9.6 |
| CC(1/2) | 1.000 | 0.800 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 1.0 uL of 20.0 mg/mL OROV Gc head domain in 20 mM Tris-Cl pH 8.0, 150 mM NaCl was added to 1.0 uL of reservoir solution containing 0.5 M lithium sulfate, 15% w/v PEG 8K |
