6H3W

La Crosse Virus Glycoprotein Gc Head Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.977170
Spacegroup name	H 3 2
Unit cell lengths	73.227, 73.227, 280.713
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.052 - 2.098
R-factor	0.1698
Rwork	0.165
R-free	0.21290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6h3x
RMSD bond length	0.007
RMSD bond angle	0.946
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.052	2.160
High resolution limit [Å]	2.098	2.100
Rmerge	0.090	1.020
Rmeas	0.090	1.090
Rpim	0.030	0.390
Number of reflections	17376	1378
<I/σ(I)>	11.4	1.8
Completeness [%]	99.3	98
Redundancy	7.9	7.6
CC(1/2)	1.000	0.650

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.75 uL of a 11.1 mg/mL protein sample in 20 mM Tris-Cl pH 8.0, 150 mM NaCl were added to 0.75 uL of reservoir solution containing 0.1 M HEPES pH7.5, 10% v/v 2-propanol, 20% w/v PEG 4K