6H3V
Bunyamwera Virus Glycoprotein Gc Head Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.033199 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 125.440, 125.440, 46.550 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.550 - 2.900 |
| R-factor | 0.1723 |
| Rwork | 0.167 |
| R-free | 0.21640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6h3w |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.155 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.550 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.090 | 1.270 |
| Rmeas | 0.090 | 1.340 |
| Rpim | 0.030 | 0.420 |
| Number of reflections | 9534 | 1519 |
| <I/σ(I)> | 16.1 | 1.9 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 9.6 | 10 |
| CC(1/2) | 1.000 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.2 uL of 22.2 mg/mL BUNV Gc head domain in 20 mM Tris-Cl pH 8.0, 150 mM NaCl were added to 0.2 uL of reservoir solution containing 0.2 M sodium acetate, 0.1 M Tris-Cl pH 8.5, 30% w/v PEG 4K |
