6H3V

Bunyamwera Virus Glycoprotein Gc Head Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033199
Spacegroup name	P 3 2 1
Unit cell lengths	125.440, 125.440, 46.550
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.550 - 2.900
R-factor	0.1723
Rwork	0.167
R-free	0.21640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6h3w
RMSD bond length	0.009
RMSD bond angle	1.155
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.550	3.080
High resolution limit [Å]	2.900	2.900
Rmerge	0.090	1.270
Rmeas	0.090	1.340
Rpim	0.030	0.420
Number of reflections	9534	1519
<I/σ(I)>	16.1	1.9
Completeness [%]	99.8	99.8
Redundancy	9.6	10
CC(1/2)	1.000	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.2 uL of 22.2 mg/mL BUNV Gc head domain in 20 mM Tris-Cl pH 8.0, 150 mM NaCl were added to 0.2 uL of reservoir solution containing 0.2 M sodium acetate, 0.1 M Tris-Cl pH 8.5, 30% w/v PEG 4K