6H3M
The crystal structure of a human seleno-insulin analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.011, 42.344, 61.453 |
| Unit cell angles | 100.58, 98.70, 117.43 |
Refinement procedure
| Resolution | 35.982 - 1.821 |
| R-factor | 0.1915 |
| Rwork | 0.190 |
| R-free | 0.22920 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.491 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.090 | 0.721 |
| Number of reflections | 28501 | 4398 |
| <I/σ(I)> | 9.67 | 1.67 |
| Completeness [%] | 96.2 | 90.7 |
| Redundancy | 5.22 | 5.04 |
| CC(1/2) | 0.996 | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.8 M NaCl, 35 mM NaCitrate, 0.5 mM ZnAcetate, 0.3 M Tris pH 7.5 |
